Analysts said the judicial interpretation specifically targeted the hundreds of Big Vs - "v" for verified - who send opinions, news and information to thousands or even millions of followers. Many are entrepreneurs, celebrities or academics.
Weibo users known for political and social commentary have been detained in recent weeks, enforcing fears that rumour-mongering is not the target of the law.
Wang Gongquan, an outspoken venture capitalist, was taken away by police on Friday on charges of disturbing public order after he helped lead a campaign for the release of another activist.
Well-known whistleblower Wu Dong, more commonly recognised by his online handle "Boss Hua", was taken into police custody in Beijing, the official Liberation Daily reported on its verified Weibo feed.
He said via Weibo on Wednesday that he had been released. His phone was off and he could not be reached for comment.
Wu, a watch lover, is one of many Weibo users who post about official corruption. He drew attention last year when he posted photos of expensive watches worn by government officials last year, helping to bring down one high-flying official.
Chinese-American venture capitalist Charles Xue was detained on charges of prostitution and appeared on state TV in handcuffs on Sunday to apologise, saying his status as a Big V had gone to his head.
"Freedom of speech cannot override the law," said Xue, who was known for outspoken comments on political and social issues online.
Spreading rumours is common on Weibo, and there is little protection against defamation.
"It's important that those who spread slander are held legally responsible," said Peng Jian, a lawyer with more than 100,000 followers on Weibo. "But if it's not implemented properly, it could suppress freedom of expression."
Opinions "shouldn't be suppressed"
"The goal is pretty obvious, and it's certainly not to suppress so-called rumours. In some sense that's just an excuse," said rights lawyer Mo Shaoping. "Most of the things people post on the Internet are just opinions and views. Those things shouldn't be suppressed."
Mo said he had stopped using Weibo two years ago under pressure from officials.
Legal experts said that the court's interpretation amounted to a broadening of authority to police the Internet, as if Weibo were a physical public space.
"It's a significant expansion because it criminalizes postings that were not criminalized before, and not only rumours and libellous comments," said Nicholas Bequelin, of New York-based Human Rights Watch.
Tong Zhiwei, a professor at East China University of Political Science and Law who has about 95,000 Weibo followers, said he had seen a rise in the number of his posts that had been censored in recent weeks.
"It's not so much the judicial interpretation per se that is affecting my comments," said Tong, who frequently comments on social issues from a legal perspective. "It's the atmosphere it has created."
Criticising one-party rule was in effect forbidden already, Bequelin added.
"Nine tenths of the Internet population would be in prison if you were to apply this very strictly," Bequelin said. "But that is the root of China's regulation of freedom of expression."
The latest crackdown was also significant because it targeted Internet users who didn't see themselves as dissidents, said Sarah Cook of Freedom House, a U.S.-based group promoting political freedom and human rights."Now you're having a criminal judicial interpretation and then actual arrests of people who don't see themselves as political," Cook said. "The group of people being targeted is much wider."