The company's shares rose as much as 8 percent to $26.21, the highest since August 2009, in morning trading on the New York Stock Exchange on Thursday.
Barnes & Noble said in June it planned to spin off Nook Digital Media, which at the time included the company's profitable and fast-growing college books unit.
"We said we were separating Nook Digital business from the retail business, but the board decided that separating the college business would allow each business to optimize the opportunities," spokeswoman Mary Ellen Keating said.
Nook will now become part of the retail business, she said.
Barnes & Noble's college book unit operates 714 stores on campuses across the United States.
The business accounted for nearly 45 percent of the company's revenue in the second quarter ended November 1.
Amazon's Kindle line of ebook readers has also eaten away market share from Barnes & Noble's Nook tablets, which have cost the company millions over the past five years.
Sales at the Nook division fell 55.4 percent to $56 million (roughly Rs. 346 crores) in the nine-week holiday period ended Jan. 3, mainly due to lower sales of tablets and accessories.
"Barnes & Noble will be able to better capitalize on improving industry trends and merchandising initiatives within its core retail business," Chief Executive Michael Huseby said.
Barnes & Noble said shares of the new company, Barnes & Noble Education, will be distributed to shareholders in a tax-free transaction expected to be completed by the end of August.
Guggenheim Securities LLC is the company's financial adviser for the spinoff.
Max Roberts, chief executive of the college bookstore unit, will lead the new company, which will be based in Basking Ridge, New Jersey.
© Thomson Reuters 2015