Ottawa-based Wi-Lan said it filed in the U.S. District Court for the Southern District of Florida against the BlackBerry maker.
Wi-Lan, which has licensed its intellectual property to more than 255 companies worldwide, alleged that RIM infringed its U.S. Patent No. 6,260,168 related to Bluetooth technologies.
Wi-Lan has launched a string of patent lawsuits, including one last week against Apple Inc, HTC Corp and Sierra Wireless Inc's U.S. unit over LTE mobile technology.
In its filing, Wi-Lan alleges that RIM's PlayBook tablet and a wide range of its smartphones, including the Bold, Torch, Pearl and Storm, utilize technology that infringes on its patent.
"RIM will vigorously defend itself against patent infringement," a spokeswoman for RIM said.
Wi-Lan is seeking an unspecified amount in damages and preliminary and permanent injunctions to block RIM from infringing its patent.Wi-Lan also said on Tuesday that the United States Court of Appeals for the Federal Circuit (CAFC) affirmed a lower court's dismissal of a patent infringement lawsuit in March against LG Electronics Inc.
The company accused LG of infringing a patent for V-chip technology, which lets parents block television content they consider inappropriate.
"Wi-Lan is surprised and disappointed by this decision. Wi-Lan is reviewing its options with counsel," the company said.
Shares of Wi-Lan were down 6 percent at C$4.53 in late-afternoon trading on the Toronto Stock Exchange.
© Thomson Reuters 2012